Safety Assessment of Agrichemicals

Ina Research Inc. supports a comprehensive range of toxicity tests required in applications for registration of new agricultural chemicals, starting from the screening stage. As a GLP-compliant, AAALAC-accredited* facility, our in vivo/in vitro tests also comply with OECD guidelines and country-specific regulations related to applications and safety assessments for new agricultural chemicals. Upon your request, we also offer consulting services on toxicity evaluation taking into account the regulations of each country.      　　　

 _{*Association for Assessment and Accreditation of Laboratory Animal Care International}

General Toxicity Tests (compliant with OECD Test Guidelines)

• OECD TG 420、OECD TG 423
                            Acute Oral Toxicity
• OECD TG 402　Acute Dermal Toxicity
• OECD TG 407　Repeated Dose 28-Day Oral Toxicity Study in Rodents
• OECD TG 408　Repeated Dose 90-Day Oral Toxicity Study in Rodents
• OECD TG 409　Repeated Dose 90-Day Oral Toxicity Study in Non-Rodents
• OECD TG 410　Repeated Dose Dermal Toxicity: 21/28-Day Study
• OECD TG 411　Subchronic Dermal Toxicity: 90-Day Study
• OECD TG 452　Chronic Toxicity Studies

　     Observations, Examinations and Measurements:
          ・Clinical observations, body weights, food and water consumption, ophthalmology, urinalysis,
            hematology, clinical chemistry, gross pathology, organ weights and histopathology
          ・Optional: auditory test, ECG, blood pressure, body temperature and thyroid-related hormones
　　　 (T3, T4, TSH)

• Analysis of dosing formulation: stability, homogeneity, verification of concentrations (solution, suspension, feed, including validation)
• Toxicokinetics: determination of test article and metabolite concentrations in biological samples (including validation)

Genotoxicity Tests (compliant with OECD Test Guidelines)

• OECD TG 471　Bacterial Reverse Mutation Test (Ames test)
• OECD TG 473　In Vitro Mammalian Chromosomal Aberration Test
• OECD TG 474　Mammalian Erythrocyte Micronucleus Test (bone marrow, peripheral blood, liver)
                             ▶ Evidence of systemic exposure can also be provided.
• OECD TG 475　Mammalian Bone Marrow Chromosomal Aberration Test
• OECD TG 487　In Vitro Mammalian Cell Micronucleus Test (TK6, CHL/IU)
                             ▶ Tests using Cytochalasin B also available.
• OECD TG 489　In Vivo Mammalian Alkaline Comet Assay (liver, stomach, bone marrow, urinary bladder)
• OECD TG 490　In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene

Reproduction and Development Toxicity Tests
(compliant with OECD Test Guidelines)

• OECD TG 414　Prenatal Developmental Toxicity Study
• OECD TG 416　Two-Generation Reproduction Toxicity
• OECD TG 421　Reproduction/Developmental Toxicity Screening Test
• OECD TG 443　Extended One-Generation Reproductive Toxicity Study (Cohort 1A, 1B, 2A, 2B)
• OECD TG 422　Combined Repeated Dose Toxicity Study with the Reproduction/Developmental
                            Toxicity Screening Test

Carcinogenicity Tests (compliant with OECD Test Guidelines)

• Short-term Carcinogenicity Studies
• OECD TG 453　Combined Chronic Toxicity/Carcinogenicity Studies

Irritation Tests (compliant with OECD Test Guidelines)

• OECD TG 404　Acute Dermal Irritation/Corrosion
• OECD TG 405　Acute Eye Irritation/Corrosion
• OECD TG 439　In Vitro Skin Irritation: Reconstructed Human Epidermis Test Methods (cultured cells)
• OECD TG 492　Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying
                            chemicals not requiring classification and labelling for eye irritation or serious eye
                            damage (cultured cells)

Sensitization Tests (compliant with OECD Test Guidelines)

• OECD TG 406　Skin Sensitisation Guinea Pig Maximisation Test and Buehler Test
• OECD TG 442B Local Lymph Node Assay: BrdU-ELISA